Ariya Lauretti Noti Pdf

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RELATED APPLICATIONS The present patent document claims the benefit of the filing date under 35 U.S.C. §119(e) of Provisional U.S.

Patent Application Ser. 60/903,116, filed Feb. 23, 2007, which is hereby incorporated by reference. FIELD The present invention relates to the field of diagnostics. More particularly, the invention disclosed herein is directed to methods, compositions, and kits for detecting nucleic acids, polynucleotides, pathogens, toxins, or other agents or factors that are desirably detected or measured.

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BACKGROUND There is a great need to detect and quantify various molecular species, such as polynucleotides, polypeptides, carbohydrates, lipids, and small molecules. For example, current methods of detecting a polynucleotide, such as those associated with pathogens, pathogen infection, human genes associated with diseases and disorders, altered physiology or physiological conditions, genetically modified organisms (GMOs, i.e., organisms with transgenic DNA), biowarfare agents, veterinary applications, and agricultural applications presently rely on complex methods, such as the polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), or branched DNA (bDNA). These methods require skilled personnel and specialized equipment.

Further, the methods are generally incapable of determining the presence or quantity of polynucleotides in crude cell and tissue extracts. There are similar difficulties in the existing immunoassays for detecting antigens.